Zuccarelli 2021 J Physiol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Zuccarelli L, Baldassarre G, Magnesa B, Degano C, Comelli M, Gasparini M, Manferdelli G, Marzorati M, Mavelli I, Pilotto A, Porcelli S, Rasica L, ล imuniฤ B, Piลกot R, Narici M, Grassi B (2021) Peripheral impairments of oxidative metabolism after a 10-day bed rest are upstream of mitochondrial respiration. J Physiol | |title=Zuccarelli L, Baldassarre G, Magnesa B, Degano C, Comelli M, Gasparini M, Manferdelli G, Marzorati M, Mavelli I, Pilotto A, Porcelli S, Rasica L, ล imuniฤ B, Piลกot R, Narici M, Grassi B (2021) Peripheral impairments of oxidative metabolism after a 10-day bed rest are upstream of mitochondrial respiration. J Physiol 599:4813-29. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/34505290 PMID: 34505290 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/34505290 PMID: 34505290 Open Access] | ||
|authors=Zuccarelli Lucrezia, Baldassarre Giovanni, Magnesa Benedetta, Degano Cristina, Comelli Marina, Gasparini Mladen, Manferdelli Giorgio, Marzorati Mauro, Mavelli Irene, Pilotto Andrea, Porcelli Simone, Rasica Letizia, Simunic Bostjan, Pisot Rado, Narici Marco, Grassi Bruno | |authors=Zuccarelli Lucrezia, Baldassarre Giovanni, Magnesa Benedetta, Degano Cristina, Comelli Marina, Gasparini Mladen, Manferdelli Giorgio, Marzorati Mauro, Mavelli Irene, Pilotto Andrea, Porcelli Simone, Rasica Letizia, Simunic Bostjan, Pisot Rado, Narici Marco, Grassi Bruno | ||
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|keywords=NIRS, PLM, Bed rest, Microgravity, Mitochondrial respiration, Oxidative metabolism, Skeletal muscle | |keywords=NIRS, PLM, Bed rest, Microgravity, Mitochondrial respiration, Oxidative metabolism, Skeletal muscle | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=IT Udine Grassi B, IT Udine Mavelli I | |||
}} | }} | ||
{{Labeling | {{Labeling |
Latest revision as of 19:00, 6 December 2021
Zuccarelli L, Baldassarre G, Magnesa B, Degano C, Comelli M, Gasparini M, Manferdelli G, Marzorati M, Mavelli I, Pilotto A, Porcelli S, Rasica L, ล imuniฤ B, Piลกot R, Narici M, Grassi B (2021) Peripheral impairments of oxidative metabolism after a 10-day bed rest are upstream of mitochondrial respiration. J Physiol 599:4813-29. |
Zuccarelli Lucrezia, Baldassarre Giovanni, Magnesa Benedetta, Degano Cristina, Comelli Marina, Gasparini Mladen, Manferdelli Giorgio, Marzorati Mauro, Mavelli Irene, Pilotto Andrea, Porcelli Simone, Rasica Letizia, Simunic Bostjan, Pisot Rado, Narici Marco, Grassi Bruno (2021) J Physiol
Abstract: In order to identify peripheral biomarkers of impaired oxidative metabolism during exercise following a 10-day bed rest (BR), 10 males performed an incremental exercise (to determine peak pulmonary VฬO2[VฬO2p]) and moderate-intensity exercises, before (PRE) and after (POST) BR. Blood flow response was evaluated in the common femoral artery by Eco-Doppler during 1-min passive leg movements (PLM). The intramuscular matching between O2 delivery and O2 utilization was evaluated by near-infrared spectroscopy (NIRS). Mitochondrial respiration was evaluated ex vivo by high-resolution respirometry in isolated muscle fibers, and in vivo by NIRS by the evaluation of skeletal muscle VฬO2(VฬO2m) recovery kinetics. Resting VฬO2m was estimated by NIRS. Peak VฬO2p was lower in POST vs. PRE. The area under the blood flow vs. time curve during PLM was smaller (P = 0.03) in POST (274 ยฑ 233 mL) vs. PRE (427 ยฑ 291). An increased (P = 0.03) overshoot of muscle deoxygenation during a metabolic transition was identified in POST. Skeletal muscle citrate synthase activity was not different (P = 0.11) in POST (131 ยฑ 16 nmol. min-1. mg-1) vs. PRE (138 ยฑ 19). Maximal ADP-stimulated mitochondrial respiration (66 ยฑ 18 pmol. s-1. mg-1 [POST] vs. 72 ยฑ 14 [PRE], P = 0.41) was not affected by BR. Apparent Km for ADP sensitivity of mitochondrial respiration was reduced in POST vs. PRE (P = 0.04). The VฬO2m recovery time-constant was not different (P = 0.79) in POST (22 ยฑ 6 s) vs. PRE (22 ยฑ 6). Resting VฬO2m was reduced by 25% in POST vs. PRE (P = 0.006). Microvascular-endothelial function was impaired following a 10-day BR, whereas mitochondrial mass and function (both in vivo and ex vivo) were unaffected or slightly enhanced. โข Keywords: NIRS, PLM, Bed rest, Microgravity, Mitochondrial respiration, Oxidative metabolism, Skeletal muscle โข Bioblast editor: Plangger M โข O2k-Network Lab: IT Udine Grassi B, IT Udine Mavelli I
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2021-09