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Pacheco 2015 FASEB J

From Bioblast
Publications in the MiPMap
Pacheco P, dos Santos V, Neves F, Bernardo A, Vieira A, Miranda G, Malafaia T, Guedes R, Moura A, Sichieri R, de Souza E (2015) Western diet induces nonalcoholic fatty liver disease and impairs liver mitochondrial metabolism in adult mice . FASEB J 29: DOI: 10.1096/fj.1530-6860.

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Pacheco P, dos Santos V, Neves F, Bernardo A, Vieira A, Miranda G, Malafaia T, Guedes R, Moura A, Sichieri R, de Souza E (2015) FASEB J

Abstract: The aim of this study was to investigate the liver mitochondrial metabolism of obese adult mice fed with Western diet (high fat diet rich in saturated fat). 21 days old male mice were divided in two groups: fed with control diet (CD) or Western diet (HFD), for 112 days. At 133 days of life the animals were sacrificed for evaluation of biometric parameters, fasting glucose, liver histology, liver mitochondrial function and proteins involved in the signaling cascade of ghrelin and insulin by Western Blotting (GHSR 1a, IRβ, PI3K, pAKT/AKT, CPT1, UCP2 and α-tubulin). Animals subjected to HFD showed increased body weight (p <0.0001), visceral fat (p <0.0001), liver weight (p <0.0001) and hyperglycemia (p <0.0133) compared to CD group. The liver of HFD group presented damaged parenchymal cells with accumulation of lipid droplets and alterations in protein content compared to CD: reduced level of GHSR1a (p <0.0282) and pAKT/AKT ratio (p <0.0053), increased content of PI3K (p <0.0149), IRβ (p <0.0539), CPT1 (p <0.0417) and decreased content of UCP2 (p <0.0196). Through high-resolution respirometry we observed a reduction in carbohydrate oxidation (p <0.0481) and increased fatty acid oxidation (p <0.0008) in the liver of HFD group. We concluded that Western diet alters energetic metabolism and impairs ghrelin signaling which favors the development of nonalcoholic fatty liver disease. Supported by FAPERJ and CNPq.


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Mouse  Tissue;cell: Liver