| Protocol |
Topic |
Has title |
| MiPNet17.02 PBI-Shredder-Manual |
mt-Preparation |
Draxl A, Eigentler A, Gnaiger E. PBI-Shredder HRR-Set: Preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02. |
| MiPNet11.05 Mitos-PermeabilizedCells |
mt-Preparation |
Gnaiger E. Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05. |
| MiPNet17.03 Shredder vs Fibres |
mt-Preparation |
Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E. Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03. |
| MiPNet08.13 MitoIsolation-RLM |
mt-Preparation |
Lassnig B, Gnaiger E. Laboratory protocol: Isolation of rat liver mitochondria. Mitochondr Physiol Network 08.13. |
| MiPNet14.14 PermeabilizedFibrePreparation |
mt-Preparation |
Pesta D, Gnaiger E. Preparation of permeabilized muscle fibres for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 14.14. |
| MiPNet06.06 ChemicalBackground |
O2k-SOP |
Kuznetsov AV, Gnaiger E. Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06. |
| MiPNet08.16 pH-Calibration |
O2k-SOP |
Gnaiger E. Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16. |
| D MiPNet12.08 O2k-Calibration |
O2k-SOP |
Gnaiger E. Oxygen and pX Calibration DatLab 4.3. Mitochondr Physiol Network 12.08. |
| MiPNet10.05 O2-Concentration-Flux |
O2k-SOP |
Gnaiger E. From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. |
| E MiPNet12.09 O2FluxAnalysis |
O2k-SOP |
Gnaiger E. Oxygen flux analysis: On-line DatLab4.3. Mitochondr. Physiol. Network 12.09. |
| G MiPNet15.03 MultiSensor-ISE |
O2k-SOP |
Fasching M, Gnaiger E. O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr. Physiol. Network 15.03. |
| MiPNet14.06 InstrumentalBackground |
O2k-SOP |
Fasching M, Gnaiger E. Instrumental background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6. |
| MiPNet06.03 O2-Calibration-Solubility |
O2k-SOP |
Gnaiger E. Oxygen calibration and solubility in experimental media. Mitochondr Physiol Network 06.03. |
| F MiPNet12.10 TIP2k-Manual |
O2k-SOP |
Fasching M, Lukas Gradl L, Gradl P, Erich Gnaiger E. Titration-Injection microPump TIP2k. User manual. Mitochondr. Physiol. Network 12.10. |
| MiPNet08.12 NO-O2kWorkshop |
O2k-Demo |
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM. NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12. |
| MiPNet08.09 CellRespiration |
O2k-Demo |
Gnaiger E, Renner K. High-resolution respirometry with cultured cells: A demonstration experiment. Oxygraph-2k workshop report, Schroecken, Austria, March 2003. Mitochondr Physiol Network 08.09. |
| MiPNet10.04 CellRespiration |
O2k-Demo |
Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04. |
| MiPNet12.23 FibreRespiration |
O2k-Demo |
Lemieux H, Votion DM, Gnaiger E. Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. |
| MiPNet14.05 TPP-MitoMembranePotential |
O2k-Demo |
Renner-Sattler K, Fasching M, Gnaiger E. TPP+ and membrane potential. Mitochondr Physiol Network 14.05. |
| MiPNet12.11 MitoRespiration |
O2k-Demo |
Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. |
| MiPNet12.17 BovineHeartMito |
O2k-Demo |
Petrosyan S, Naviaux R, Haas RH, Gnaiger E. Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17. |
| MiPNet11.09 MitoPathways-CII |
MitoPathways |
Gnaiger E. Mitochondrial pathways to Complex II, glycerophosphate dehydrogenase and electron transferring flavoprotein. Mitochondr Physiol Network 11.09. |
| MiPNet12.12 MitoPathways-CI+II |
MitoPathways |
Gnaiger E. Mitochondrial pathways through Complexes I+II: Convergent electron transport at the Q-junction and additive effect of substrate combinations. Mitochondr Physiol Network 12.12. |
| MiPNet11.04 MitoPathways-CI |
MitoPathways |
Gnaiger E. Mitochondrial pathways to Complex I: Respiration with pyruvate, malate and glutamate. Mitochondr Physiol Network 11.04. |
| MiPNet12.15 RespiratoryStates |
MitoPathways |
Gnaiger E. MitoPathways: Respiratory states and flux control ratios. Mitochondr Physiol Network 12.15. |
| MiPNet08.14 CitrateSynthase |
Enzymes |
Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr. Physiol. Network 08.14. |
| MiPNet08.18 LactateDehydrogenase |
Enzymes |
Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr. Physiol. Network 08.18. |
| MiPNet08.15 Complex-I |
Enzymes |
Kuznetsov AV, Gnaiger E. Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochondrial membrane enzyme. Mitochondr. Physiol. Network 08.15. |
| MiPNet14.13 Medium-MiR06 |
Chemicals & Media |
Fasching M, Renner K, Gnaiger E. Mitochondrial respiration medium - MiR06. Mitochondr Physiol Network 14.13. |
| MiPNet03.02 Chemicals-Media |
Chemicals & Media |
Lassnig B, Stadlmann S, Rieger G, Haffner B, Lemieux H, Gnaiger E. Selected media and chemicals for respirometry with mitochondria and permeabilized cells. Mitochondr Physiol Network 03.02. |
| MiPNet09.12 O2k-Titrations |
Chemicals & Media |
Lemieux H, Gnaiger E. Oxygraph-2k titrations: Mitochondria, permeabilized cells and biopsies. Mitochondr Physiol Network 09.12. |