OroboroPedia: Protocols

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O2k-Protocols

Protocol Topic Has title
MiPNet17.02 PBI-Shredder-Manual mt-Preparation Draxl A, Eigentler A, Gnaiger E. PBI-Shredder HRR-Set: Preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02.
MiPNet11.05 Mitos-PermeabilizedCells mt-Preparation Gnaiger E. Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05.
MiPNet17.03 Shredder vs Fibres mt-Preparation Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E. Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03.
MiPNet08.13 MitoIsolation-RLM mt-Preparation Lassnig B, Gnaiger E. Laboratory protocol: Isolation of rat liver mitochondria. Mitochondr Physiol Network 08.13.
MiPNet14.14 PermeabilizedFibrePreparation mt-Preparation Pesta D, Gnaiger E. Preparation of permeabilized muscle fibres for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 14.14.
MiPNet06.06 ChemicalBackground O2k-SOP Kuznetsov AV, Gnaiger E. Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06.
MiPNet08.16 pH-Calibration O2k-SOP Gnaiger E. Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16.
D MiPNet12.08 O2k-Calibration O2k-SOP Gnaiger E. Oxygen and pX Calibration DatLab 4.3. Mitochondr Physiol Network 12.08.
MiPNet10.05 O2-Concentration-Flux O2k-SOP Gnaiger E. From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05.
E MiPNet12.09 O2FluxAnalysis O2k-SOP Gnaiger E. Oxygen flux analysis: On-line DatLab4.3. Mitochondr. Physiol. Network 12.09.
G MiPNet15.03 MultiSensor-ISE O2k-SOP Fasching M, Gnaiger E. O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr. Physiol. Network 15.03.
MiPNet14.06 InstrumentalBackground O2k-SOP Fasching M, Gnaiger E. Instrumental background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6.
MiPNet06.03 O2-Calibration-Solubility O2k-SOP Gnaiger E. Oxygen calibration and solubility in experimental media. Mitochondr Physiol Network 06.03.
F MiPNet12.10 TIP2k-Manual O2k-SOP Fasching M, Lukas Gradl L, Gradl P, Erich Gnaiger E. Titration-Injection microPump TIP2k. User manual. Mitochondr. Physiol. Network 12.10.
MiPNet08.12 NO-O2kWorkshop O2k-Demo Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM. NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12.
MiPNet08.09 CellRespiration O2k-Demo Gnaiger E, Renner K. High-resolution respirometry with cultured cells: A demonstration experiment. Oxygraph-2k workshop report, Schroecken, Austria, March 2003. Mitochondr Physiol Network 08.09.
MiPNet10.04 CellRespiration O2k-Demo Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.
MiPNet12.23 FibreRespiration O2k-Demo Lemieux H, Votion DM, Gnaiger E. Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23.
MiPNet14.05 TPP-MitoMembranePotential O2k-Demo Renner-Sattler K, Fasching M, Gnaiger E. TPP+ and membrane potential. Mitochondr Physiol Network 14.05.
MiPNet12.11 MitoRespiration O2k-Demo Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11.
MiPNet12.17 BovineHeartMito O2k-Demo Petrosyan S, Naviaux R, Haas RH, Gnaiger E. Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17.
MiPNet11.09 MitoPathways-CII MitoPathways Gnaiger E. Mitochondrial pathways to Complex II, glycerophosphate dehydrogenase and electron transferring flavoprotein. Mitochondr Physiol Network 11.09.
MiPNet12.12 MitoPathways-CI+II MitoPathways Gnaiger E. Mitochondrial pathways through Complexes I+II: Convergent electron transport at the Q-junction and additive effect of substrate combinations. Mitochondr Physiol Network 12.12.
MiPNet11.04 MitoPathways-CI MitoPathways Gnaiger E. Mitochondrial pathways to Complex I: Respiration with pyruvate, malate and glutamate. Mitochondr Physiol Network 11.04.
MiPNet12.15 RespiratoryStates MitoPathways Gnaiger E. MitoPathways: Respiratory states and flux control ratios. Mitochondr Physiol Network 12.15.
MiPNet08.14 CitrateSynthase Enzymes Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr. Physiol. Network 08.14.
MiPNet08.18 LactateDehydrogenase Enzymes Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr. Physiol. Network 08.18.
MiPNet08.15 Complex-I Enzymes Kuznetsov AV, Gnaiger E. Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochondrial membrane enzyme. Mitochondr. Physiol. Network 08.15.
MiPNet14.13 Medium-MiR06 Chemicals & Media Fasching M, Renner K, Gnaiger E. Mitochondrial respiration medium - MiR06. Mitochondr Physiol Network 14.13.
MiPNet03.02 Chemicals-Media Chemicals & Media Lassnig B, Stadlmann S, Rieger G, Haffner B, Lemieux H, Gnaiger E. Selected media and chemicals for respirometry with mitochondria and permeabilized cells. Mitochondr Physiol Network 03.02.
MiPNet09.12 O2k-Titrations Chemicals & Media Lemieux H, Gnaiger E. Oxygraph-2k titrations: Mitochondria, permeabilized cells and biopsies. Mitochondr Physiol Network 09.12.
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