O2k versus multiwell respirometer

From Bioblast

Jump to: navigation, search


OROBOROS         Products         Support         O2k-Publications         Workshops & Events         O2k-Network         Feedback         Company         Contact

O2k versus multiwell respirometer

O2k-Catalogue» OROBOROS O2k-Catalogue, Order @OROBOROS

Description O2k versus multiwell respirometer: O2k stands for Oxygraph-2k and high-resolution respirometry, meeting powerful quality criteria securing high output and pioneering state-of-the-art comprehensive OXPHOS analysis of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target (Silver 2012 Penguin Press).
Product ID * Open Access: O2k vs multiwell.pdf
Type O2k, Feedback
Link Bioblast pdf

MitoPedia O2k and high-resolution respirometry: O2k hardware 

Publications in the MiPMap
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.

» Bioblast pdf

OROBOROS (2015-05-21) MiPNet

Abstract: Gnaiger E, Fasching M (2015) O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(05): 1-8. » Versions

O2k-Specifications O2k-Network Lab: AT Innsbruck OROBOROS

Labels: MiParea: Respiration, Instruments;methods Mammal;model: Human  Cell line: Fibroblast Preparation: Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP HRR: Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory 


Scientists who have experience with both instruments are invited to extend the discussion:
» Microwell respirometry
» Tony Hickey: Calibration is my chief concern
» .. and more: Talk:O2k versus multiwell respirometer
» XFe for isolated mitochondria - a critical discussion

Selected related links

» Low running costs for the OROBOROS O2k
» Power-O2k
» O2k-Publications: Topics
» More details on O2k-Specifications? Consult OROBOROS

Conflict of interest

Erich Gnaiger is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.

Selected references

  • Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. »Bioblast pdf«
  • Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. »Bioblast pdf«
  • Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. »Open Access«
  • Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. »Bioblast pdf«
  • Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. »Open Access«
  • Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. »Open Access«
  • Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. »Open Access« </ref>
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast pdf«
  • Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. »Bioblast pdf«
  • Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. |»Bioblast pdf«
  • Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. |»Open Access«
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast pdf«
  • Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Bioblast pdf«
Personal tools
Support pages