O2k-Feedback

From Bioblast

Description	O2k-Feedback: OROBOROS - Cooperation and Feedback in Science
Product ID	*****-++
Type	O2k, Feedback, Catalogue
Link	http://www.oroboros.at/?HRR-Feedback
Image

Contents 1 O2k-Feedback 2012 1.1 IOC68 O2k-Workshop Schroecken, April 2012 1.2 IOC66 O2k-Fluorometry Workshop Innsbruck, March 2012 2 O2k-Feedback 2011 3 O2k-Feedback 2010 4 O2k-Feedback 2009 5 O2k-Feedback 2008 6 O2k-Feedback 2007 7 O2k-Feedback 2006 8 O2k-Feedback 2005 9 O2k-Feedback 2004 10 O2k-Feedback - specific topics

O2k-Feedback 2012

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IOC68 O2k-Workshop Schroecken, April 2012

• Thank you so much for the wonderful time in Schröcken (IOC68). It was, by FAR, the best workshop/conference/meeting that I have had the pleasure of attending. The balance between demonstration and hands-on experience was perfect. I cannot overstate the tremendous hospitality at Berghotel Körbersee, as well as the friendly, and fun environment of Gentle Science! ... admittedly the unparalleled company, sun, snow, scenery, and copious volume of weissbier were my favorites! Cannon DT, Leeds, UK; MiPNet Lab: US CA Torrance Rossiter HB (2012-04-17)
• We greatly appreciate a wonderful time in Schrocken and we hope to meet you in the near future. Olek R MiPNet Lab: PL Gdansk Kaczor JJ (2012-04-18)
• It was the best workshop or course I attended during my PhD so far. And I will closely follow your progress with the homogenizer since I see the great potential it has. I guess everyone that has isolated mitochondria would. Lund J, MipNet Lab: NO Tromso Larsen TS (2012-04-18)
• I think I was just overwhelmed by all the excellent experiences from the wonderful workshop. I started to set up my brand new O2k today, very eager to get it work as soon as possible. It is such an honor to have the chance to share your experience with O2k and to get the intelligence answers of lots of my questions from you. I feel myself so motivated to enjoy the complex and mysterious world of mitochondria/bioblast. I will be very proud to see the success of Oroboros worldwide, and I do hope that I can contribute to that! Li Y, MiPNet Lab: SG Singapore Summers SA (2012-04-20)
• The IOC68 was really great!!! The Oroboros team, the program and location of the workshop, and the nice and enthusiastic people attending; something very, very special!! I enjoyed and learned a lot! Arbo I, MiPNet Lab: NO Trondheim Rognmo O (2012-04-20)
• I really enjoyed the IOC68 workshop. Thanks for the opportunity to take part. I truly learned a lot and look forward to the potential of attending a summer session and/or becoming a guest tutor. Woodlief TL, MiPNet Lab: US PA Pittsburgh Goodpaster BH (2012-04-23)
• Thank you for this great time at this wonderful place, it was a big pleasure for me to take part. I really learned a lot and it was great to meet so nice and interesting people from all over the world. Thanks to the whole Oroboros Team! Schmitz L, MiPNet Lab: DE Cologne Larsson NG (2012-04-28)
• IOC68 Photos

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• It was a great pleasure to join the O2k-Workshop Sydney and meet you there. I enjoyed every section of your speech, they are all wonderful! I also enjoyed the first dinner with you, the chat was excellent. Lanfeng Dong (2012-04-06)
• It was really great meeting you in Sydney the other week at the Oroboros workshop. I learnt a lot about mitochondrial respiration. Angelina Y Fong, Australian School of Advanced Medicine (2012-04-27)

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IOC66 O2k-Fluorometry Workshop Innsbruck, March 2012

The discussion continues on 'Oxygen dependence of mt-ROS production and evaluation of optimum experimental models':

--> Talk:Permeabilized muscle fibres

• I want to thank you for a fantastic IOC66 workshop and encourage you to continue with your genuine view of science and quality product design and implementation! Garcia-Roves PM, MiPNet Lab: ES Barcelona Garcia-Roves PM (2012-03-27)

• Thank you for last week's workshop. I greatly enjoyed meeting and talking with you, and with others attending the workshop. I was very impressed with the new fluorometry measurements in the O2K – it was really excellent to see the results real-time during the workshop. I greatly appreciate your commitment to the highest quality with regard to equipment and data analysis, and also greatly appreciate your personal commitment to train bioenergeticists in such workshops. It is unique internationally! Dr. Mary-Ellen Harper, University of Ottawa, Canada (2012-03-19)

• I would like to thank you for the excellent meeting last week. I really enjoyed the discussions and the demonstration experiments were very informative. Thanks again for all your work in organising the meeting and I hope that you enjoyed it as much as I did! Boyle JP, MiPNet Lab: UK Leeds Peers C (2012-03-19)

• It was a great pleasure to participate at the IOC66. I think it was received very well by all participants, including my students and myself! Chinopoulos C, MiPNet Lab: HU Budapest Chinopoulos C (2012-03-19)

• Thank you very much for the wonderful time you and your team gave me (us) in Innsbruck. I am looking forward to continue our cooperation! Tretter L, MiPNet Lab: HU Budapest Tretter L (2012-03-18)

__________

• Thank you for creating such a wonderful apparatus, it made my work so much easier and much more fun. Thanks also for a great course in Schroecken in the spring of 2009, unforgettable! Persson MF, MipNet Lab: SE Uppsala Liss P (2012-03-05)

• DatLab is a fabulous software - so easy to use. Palm_F at 2nd Innsbruck-Uppsala MiPNet-Workshop, MiPNet Lab: SE Uppsala Liss P (2012-03-01)

• I had not expected that you would send the replacement stoppers before we had sent the others back. This goes above and beyond excellent customer service, you are fantastic! (2012-01-31) Chris Hedges, Victoria University, Australia - Nigel Stepto's MipNet Lab

O2k-Feedback 2011

• With my very best wishes of a productive 2012 and keep moving on the Oroboros to new horizons, bringing science, personal and life quality as driving forces to a better world. Marcus Fernandes de Oliveira, Rio de Janeiro, BR (2011-12-29)

• It is great that you develop the Oxygraph-2k in so wonderful directions.. excellent :))). Magda Labieniec-Watala, Lodz, PL (2011-11-25)

• I also just want to mention how happy we are with the O2k respirometer we have and want to congratulate you on the success of your O2k systems (not surprisingly, of couse), hence the already booked December workshop. Catherine Le, Fort Collins, US (2011-11-11)

• The first Oroboros in our lab gave good results. We have plans to purchase a second Oroboros. Daphna D.J. Habets Maastricht, NL (2011-11-04)

• Actually, we are very satisfied with the results that we are producing with the Oxygraph-2k. Silvia Cadena, Curitiba - Paraná, Brasil (2011-10-11)

• We are really satisfied with the O2k and we hope to measure mitochondrial membrane potential ASAP. Siamak Djafarzadeh, Bern, CH (2011-09-16)

• Thank you for developing the Oxygraph, it has proved to be an excellent tool and we look forward to upgrading in the near future. Campbell W. Gourlay, Kent, UK (2011-08-18)

• Our two Oroboros machines function perfectly, and I have put in a grant for a third machine. Carsten Lundby, Zurich, CH (2011-08-03)

O2k-Feedback 2010

• Our O2k runs without problems. Wolfgang Burgstaller, Innsbruck (2010-11-04)

• I was participating last summer in WorldPharma 2010 congress in Copenhagen and heard a fantastic presentation given by Professor Sir Salvador Moncada about mitochondrial function measured by OROBOROS Oxygraph. Eero Mervaala, Helsinki (2010-10-19)

• Our project is now accepted by the French polar institute (IPEV) and we plan to install a respirometry system in Antarctica! I already worked on the Oroboros in my previous laboratory in Canada and, to my opinion, it’s the best and more accurate system for our project. Caroline Romestaing, Villeurbanne (2010-09-15)

• Recently Doug Crawford here in Miami let me use his O2k and it was great. Carlos Moraes, Miami cmoraes@med.miami.edu (2010-05-05)

• The two recently acquired Oxygraphs are now installed and running smoothly. I am very pleased with the new version of Datlab, even though I have not yet had occasion to try out all of the new options added to it. Ib Therkelsen, Copenhagen, Denmark (2010-03-17) Comment: the Quistorff lab has now a total of five O2k instruments.

• We´re very happy with our three O2k's here at Karolinska Institute. Filip Larsen, Stockholm, Sweden (2010-03-05)

• Happy new year to you and the fantastic Oroboros team! The Oxygraph-2k is working like a dream. Vinh Thanh Phung, Ås, Norway (2010-01-07)

O2k-Feedback 2009

• I already start to use this wonderfull instrument. It works really well now! Arnaud Mourier, Max Planck Institute for Biology of Ageing, Zentrum für Molekulare Medizin, Univ. Köln, Germany (2009-11-16)

• I came across your website and found excellent information for instrumentation, protocols and solutions. Steven Dworetzky, Knopp Neurosciences, USA (2009-10-01)

• .. we are very impressed with the instrument .. Marni J. Falk, The Children's Hospital of Philadelphia, Philadelphia, USA (2009-08-11)

• ... For an independent perspective on the OROBOROS Oxygraph-2k, we think they are fantastic, fullstop. We have had no real issues in almost four years. I started with the general system which requires water baths etc and really it was baptism of fire, and when we got the Oroboros-O2k it was a different world. Our only issue now is we need more of them now. Perhaps the best part of our dealings with OROBOROS is Erich's groups outstanding efforts to provide detailed methods and protocols for both the machines and experiments. ... Anthony J.R. Hickey, University of Auckland, New Zealand (2009-08-07)

• ... regarding the Seahorse ... they were adament that their instrument was useful for quantitative measurements, even though the vast majority of the data was expressed as fold-change, or % control. In the end, they said that their system was more complimentary to existing oxygraphs, and even suggested that the Seahorse be used for optimization experiments to determine the best conditions for O2k experiments. Daniel A. Kane, East Carolina University, Greenville, NC, USA (2009-07-30)

• First of all I would like to write that O2K works very well in our lab as well. Laszlo Tretter, M.D., PhD. Assoc. Prof., Semmelweis University, Dept. Medical Biochemistry, Budapest, Hungary (2009-07-29)

• First of all I´d like to say that I´m very happy with the O2k system. I have used it quite a lot lately, mainly doing phosphorylation control titrations with intact podocytes and I have not have any problems, except for a few small ones relating to software. Ilkka Miinalainen, Ph.D., NICB, Centre for Bioanalytical Sciences, Dublin City University, Ireland (2009-04-27)

• We continue to "spread the word" about the OROBOROS with our colleagues at meetings and conferences. As with all quality products, I'm not surprised to hear that demand is steady, even in the face of economic hardships. Yours has the added benefit of sophisticated science and precision manufacturing, which exponentially increases its value. Darrell Neufer and I have a big paper being published in JCI on this upcoming Monday (on-line first). There is OROBOROS data all over it. Many more papers are currently being written, as we've been using our O2k's at maximal capacity for the past two years! Would it be possible for you to send a small stack of your "Blue Book of Mitochondria" with the new O2k? It's really an invaluable resource for students and new people that join the lab. Ethan J. Anderson, Ph.D., Research Associate, Departments of Cardiovascular Sciences/EXSS, Brody School of Medicine, East Carolina University, USA (2009-01-30)

• Since the two OROBOROS installed here are fully operational (in fact it has been a great success!), the demand for their use has increased susbtantially. Therefore, we are intending to purchase another one. Marcus F Oliveira, PhD, Instituto de Bioquímica Médica (IBqM), Universidade Federal do Rio de Janeiro, Brasil (2009-01-28)

O2k-Feedback 2008

• I thought you might be interested to know more about my research and my success stories using your OROBOROS. I investigated mitochondrial capacity in cardiac muscle fibers of a common model of dietary-induced insulin resistance mice and successfully presented parts of my results at the 2008 Annual Obesity Meeting, Phoenix, Arizona. I received so many positive feedbacks on my study. I am planning on developing my area of research from animals to humans, if it would be possible. I am very proud of what I have achieved during the last year. Starting with basic knowledge about mitochondria and OROBOROS, I have been able to find my way through the mitochondria respiratory world. This achievement wouldn’t be possible without your help and support. - Zahra Ezzat Zadeh, University of Calgary, Canada (2008-12-23)

• The assemblage and tests of O2K have been finished and some trials have been conducted for TCM research. - Canghai Li, China Academy of Chinese Medical Sciences, Beijing, P.R.China (2008-10-23)

• The second O2K works perfectly. It will really help us to develop more quickly applied respirometry on muscle cell culture and biopsies.' - Dominique Votion, Faculty of Veterinary Medicine, University of Liège, Berglium

• 'I have several papers in the pipeline that utilize respiration as our primary variable. The Oxygraph-2k was one of the best investments I ever made.' - James Bennett, Department of Neurology, University of Virginia, Charlottesville, USA (2008-09-03)

• 'The results with the lymphocytes cells are excellent with MiR05 and really better than with the other medium (more stability and higher values).' - Virginie Rhein, Neurobiology Lab for Brain Aging and Mental Health, Associated Research Group Dept. Biomedicine Univ. Basel, Psychiatric University Clinics Basel, CH (2008-08-29)

• 'I have been studying respiration in cells and in animals, and plan to include mitochondria. Your website will be a valuable resource. My students will find it interesting.' - Dennis Searcy, Professor of Biology, University of Massachusetts

• 'SUPERSATISFIED!' - Eskil Elmer, Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, Lund, Sweden (2008-06-25)

• 'Our OROBOROS Oxygraph is in almost daily use and we are very pleased with the data it produces." - Paula M. Keeney, Department of Neurology, University of Virginia, Charlottesville, USA.

• 'Our new O2k started up perfectly. The new software is fantastic too." - Anthony Hickey, School of Biological Sciences, University of Auckland, New Zealand.

• 'I would like to congratulate the Oroboros team for the software that really works beautifully. People who did it are really clever (know how someone using an oxygraph works)." - Frédérick Bouillaud, Faculté de Médecine Necker-Enfants Malades, Paris, France.

• 'People are getting really happy with the Oroboros ... the instrument is working remarkably well so far." - Rafael Radi, Universidad de La Republica, Montevideo, Uruguay.

• '.. we tested a few techniques using the O2K. I really enjoyed working with the instrument and so far I am very happy with it." - Charles-A. Darveau, Dept. of Biology, University of Ottawa, Canada.

O2k-Feedback 2007

• '.. feedback about the O2K. I am very impressed. The last 3 years I've done many expts. on mito respiration in permeabilized fibers using a Hansatech electrode, which worked fine and was suitable for our needs at the time. But I equate going from that system to the O2K to starting with a Honda Accord and going to an Infiniti or a Mercedes C-class. Once you've had a taste of high-performance, there's no going back. :)" Ethan J. Anderson, East Carolina University, NC, USA

• 'We are loving work with the OROBOROS-O2k, despite we were not using all possibilities yet. But, step by step..." Antonio Galina, Inst. Med. Biochem., Federal Univ. Rio de Janeiro, Brazil

• 'Our Oxygraph-2k is in daily use and is a truly wonderful instrument. I may be forced to buy a second one. - I am very interested in the TPP+ electrode development. Please keep us in mind as a potential "beta" testing site." James P. Bennett, Center for the Study of Neurodegenerative Diseases, Charlottesville, VA, USA

• 'I have been working with CS using your protocol (Laboratory Protocol Citrate Synthase), which I found to be extremely helpful and very well-written. Thank you, very good job!" Nikolai Chepelev, M.Sc. Biology Student, Carleton University, Ottawa, Canada

• 'Your web page provides the clearest, most concise explanations I have ever read on why mitochondrial substrates are used as they are (glutamate, malate, etc...). Florian L. Muller, Ph.D., Dept. of Cell and Structural Biology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA

• 'We had no problems with installation of the Oxygraph-2k." Olivier Douay, Univ. Hospital Angers, France - (2007-03-27)

• 'The O2k arrived Friday last week in excellent condition, IT IS A BEAUTY! We have installed the software and set up the electrodes and everything seems to work nicely." Eskil Elmér Lund University, Sweden (2007-01-15)

O2k-Feedback 2006

• See also O2k-course IOC32-Feedback.

• 'We are working hard with the excellent Oxygraph-2k. .. I am spreading the good news about your perfect oxygraph. I've made a collegue of mine enthusiastic, ... Willem Sluiter - w.sluiter@erasmusmc.nl (2006-10-18)

• 'Concerning our three Oxygraph-2k, they are all running smoothly like Swiss clocks (Austrian clocks, if you like...). My college, Morten, who also joined the IOC32 in April 2006, has been using the instruments almost every day through the entire summer for some permeabilised muscle experiments. He reports that all three have been pleasantly stable and well-behaven, and that electrode-membrane replacements have been rare. Only weak spot seems to be the cable connection in the bottom of the electrode holder. The cable does not like to be touched, bent, or moved about during data acquisition. Some stabilisation of the cable entry into the electrode holder might possibly be a point for improvement in later generation design. Last week of August, we took the three instruments to the nearby veterinarian school in order to perform measurements on 40 biopsies from sheep. Those were busy days for all three persons involved, but also quite satisfactory, as there was not a single mishap with either samples or machines. As to the Savitzky-Golay filter [Oxygen Flux and Time Resolution] and two second sampling intervals, we have found that for all experiments performed in our lab up until now, we never needed to do it any differently. We have not yet had the need for rapid transition measurements, but we have tried, once, to run the data acquisition at full pelt, just for the fun of it." Ib Therkelsen, University of Copenhagen, Denmark (2006-10-17)

• '... Now my Ok2 is operating soooooooooo well, and the problem of analysis of the mito data has been solved successfully as you said me [Oxygen Flux and Time Resolution]. I reduced the mitochondrial concentration (from 0.5 to 0.3 mg protein/ml ) and I´ve reduced the data recording interval to the minimum of 0.2 s too. After ADP additon the slope increases sharply and returns to state 4 with similar values to State 2 ones, resulting in a higher RCR." Ana López Ramírez - anillalopezramirez@hotmail.com (2006-10-17)

• 'Yes the machine is working well. Very well actually. Took a bit of trial and error with the electrolyte, as our water here is full of metal ions. Distilled water did the job." Anthony J.R. Hickey, University of Auckland, New Zealand (2006-07-13)

• 'We are delighted with our O2k! It is obviously a very sensitive piece of equipment and the DatLab software makes using it very easy. The respiratory state of our cell lines will be important information for our group. I must be honest though, with no background in respirometry or with the O2k, I would have been lost without all the very helpful instruction I received in Shrocken. Thank you for making our use of this fine machine possible!" Paula Keeney, Center for the Study of Neurodegenerative Diseases, Charlottesville, USA (2006-06-02)

• 'Here we are! We received the Oxygraph-2k on wednesday, we tested it for two days and it workes very well! Great!" Mamen Carmona, Diabetes Laboratory, Barcelona, Spain (2006-02-24)

• The following review of one of your products has been left by a SelectScience.net member (2006-02-09):

Product: Oxygraph-2k

Ease of use: 5 (top rating)

After sales: 5 (top rating)

Value for money: 5 (top rating)

Overall: 5.0 (top rating)

Comments: Easy to use, with a user-friendly interface. Online results are great!

Name: Rasmus Rabol - rasmusr@mfi.ku.dk

Company: University og Copenhagen

Field: Med Physiology

O2k-Feedback 2005

• On respirometry with permeabilized muscle fibres: .. thanks for your help and lifesaving website and info. Anthony J.R. Hickey, University of Auckland, New Zealand (2005-12-08)

• 'Getting OROBOROS was a natural choice." Hong Kyu Lee, Seoul National University Hospital, South Korea (2005-11-25)

• 'We have an Oroboros-2k which is a perfect machine. On the web-site you will find all material relevant to this machine, including mito isolation protocols and an estimate of the amount of tissue needed. The Oroboros simultaneously runs respirometry in 2 two ml chambers. The large volume is advantageous as this implies your mitos can respire for quite a while without running out of oxygen. As the sensors are fairly sensitive and stable you do not really need much tissue. ... you may consider to run the assays not on isolated mitos but on permeabilized cardiomyocytes (we run analyses on permeabilized muscle biopsies ..). The advantage is that this may need less tissue; .. the machine can do wonderful things. If you decide to buy one I can certainly advise you to run the course Erich offers as it is by far the best way to get some feeling of what can be achieved with the machine." Matthijs Hesselink, Maastricht University, The Netherlands (2005-11-22)

• 'The perspectives of working with the OROBOROS Oxygraph-2k on human tissue are enormous." Flemming Dela, Department of Medical Physiology, The PANUM Institute, Copenhagen, Denmark (2005-11-21)

• 'It is a pleasure to give you the permission to list our lab as an OROBOROS 'Mitochondrial Physiology Network Laboratory'. I do think this kind of network will be very useful for us." Louis Lefaucheur, Agrocampus Rennes Systèmes d'Elevage et Nutrition Animale et Humaine, Saint-Gilles, France (2005-10-27)

• 'Polarography is a technique that we have been using routinely in the lab for mitochondrial research or the diagnosis of mitochondrial diseases, and so far the Oroboros Oxygraph is the best in terms of sensitivity and reproducibility. Respiratory studies with cells give good results with about 5 10E5 cells/ml , and depending on the cell type you can go down to 1E5. We used primary myoblasts, keratinocytes, lymphocytes and a variety or cancer cells, permeabilized or not. You can also utilize permeabilized muscle fibers of about 3 mg each. The good point is that you can measure the oxygen consumption of the electrode (noise) and the oxygen diffusion inside the machine in a pre-experiment, that you remove from your respiration data. For kinetic studies you can also take into account the time constant response of the electrode (that increases with time) that can also be measured. What must be pointed out, is that this Oxygraph is the only one that allows to work at very low (physiological and even hypoxic) oxygen concentrations, what cannot be performed with classic systems that are not oxygen-proof enough. Also, the software is powerful and the new version more user-friendly as older versions. Recently, this company developed a new stopper that allows to fit in electrodes or optic fibers that allow to measure bioenergetic or cellular parameters concomitantly to oxygen consumption rate.' Rodrigue Rossignol (MIG list circular 2005-04-28)

• 'I have been working with the Oroboros Oxygraph-2k since two years now, and concerning the sensitivity I can say, that I obtained reliable results even with cell numbers of about 0.1 Mill cells/ml, which would correspond to a cellular protein concentration of about 0.3 mg/ml." Evi Hütter (MIG list circular 2005-04-28)

• 'I have been using the Oxygraph-2k for two years and due to its sensitivity I can use it to measure the respiration of intact fibroblasts from one 75 sq.cm culture flask - that could be like 0.2 mg/ml of protein and I guess it might be possible to go even lower with the protein concentration. With isolated mitochondria, measurements at 0.025 mg/ml are feasible. I must say I am very satisfied with the instrument, but frankly I have never used any other oxygen electrode so my opinion is not unbiased." Petr Pecina (MIG list circular 2005-04-27)

O2k-Feedback 2004

• 'Your new machine is amazingly confortable to work with. Time constants are really good and the new software used to drive the machine is more convenient than the previous DatLab 2 acquisition program ! We have very nice results on skinned fibers from pig or rat heart. Signals and biological samples are really stable over a large time scale, which is needed to perform accurate ADP titrations. We would like to buy another OROBOROS Oxygraph 2k. This would allow two persons of the lab to perform large scale and simultaneous studies on isolated mitochondria, myocardic cells and skinned fibers." Bertrand Beauvoit (2004-07-07)

• 'This is to express our sincere gratitude for the course last weekend. We found the course highly instructive and fruitful and have enjoyed the relaxed atmosphere very much." Matthijs Hesselink (2003-12-16)

• 'The OROBOROS precision for O2 concentration measurements is 0.036 microM against 0.500 to 1.000 microM for other commercial instruments." Grégori G, Denis M, Lefèvre D, Beker B (2002)

O2k-Feedback - specific topics

Title	Description	Product id	Product image
O2k-Feedback	O2k-Feedback: OROBOROS - Cooperation and Feedback in Science	*****-++
O2k versus multiwell respirometer	O2k stands for Oxygraph-2k and high-resolution respirometry, meeting powerful quality criteria thus securing high output. 'High throughput' stands for multiwell systems. In respirometry, this is not equivalent to high output.	*****---