MiR05Cr

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MitoPedia

MiR05Cr

Description

Mitochondrial respiration medium, MiR05Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.

MiR05Cr = MiR05 + 20 mM creatine.

Abbreviation: MiR05Cr

Reference: MiPNet14.13






MitoPedia topics: Media for respirometry 

  • For preparation of MiR05Cr and application in HRR, see MiPNet14.13 Medium-MiR06
  • The oxygen solubility factor of MiR05Cr is the same as for MiR05 = 0.92.


Rationale: as for MiR05, but less ADP is needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.