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Trondheim NO, 2013 Dec 10-11, OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, OROBOROS IOC82. Satellite to the 5th Seminar on Exercise in Medicine. Trondheim, Norway, O2k-Network Lab »NO Trondheim Rognmo O.
The 82nd Workshop on high-resolution respirometry and O2k-Fluorometry is the first Oxygraph-2k Workshop held in Norway. Most participants are experienced in high-resolution respirometry (HRR). The O2k-Workshop includes a basic introduction to quality control of instrumental performance of the OROBOROS O2k with integrated on-line analysis, introducing new features of DatLab 5.2. »Versions
The main focus will be a discussion on optimization of OXPHOS analysis in various mitochondrial (mt) preparations (permeabilized muscle fibres, tissue homogenate, isolated mitochondria). HRR provides information on cell respiration with simple phosphorylation control protocols. State-of-the-art OXPHOS analysis is extended using mt-preparations, to evaluate coupling efficiencies and OXPHOS capacities with carbohydrate versus fatty acid substrates, and to diagnose defects in respiratory complexes of the electron transfer system and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement of hydrogen peroxide production (Amplex red®) or mt-membrane potential (Safranin). Discussions are extended on comparison of measurement of mt-membrane potential using Safranin (fluorometric) versus TPP+ or TPMP+ (potentiometric), and on perspectives of HRR in mitochondrial physiology.
The Cardiac Exercise Research Group (Norwegian University of Science and Technology, Trondheim) is a MiPNet Lab offering their facility and O2ks for the training at the O2k-Workshop. The MiPNet Lab NO_Trondheim_Rognmo_O uses three O2k-instruments as a Power-O2k Laboratory.
Labels: HRR: Oxygraph-2k Additional: ORO, IOC, 2013, O2k-Network
Special evening: Man in Extreme Environments
Summary of the Man in Extreme Environments-Evening: Christopher McDougall - Born to Run
- This workshop is a component of the K-Regio project MitoCom.