MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver

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O2k-Protocols
Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver.

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OROBOROS (2015-06-16) Mitochondr Physiol Network

Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6. »Versions

A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS O2k, O2k-Catalogue

Keywords: Tissue homogenate, Shredder

O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom


Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Heart, Nervous system, Liver  Preparation: Homogenate 


Coupling state: LEAK, OXPHOS, ETS  Pathway: N, S, NS  HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol 

Additional: Mt-Preparation, PBI-Shredder 

Further information

>> PBI-Shredder HRR-Set