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Hyderabad IN, 2011 Dec 08. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) - Satellite to 1st SMRM, IOC64. »IN Hyderabad Thangaraj K.
Abstract: International Course on High-Resolution Respirometry - Satellite to 1st SMRM. Hyderabad, India; 2011 December 08
Additional: ORO, IOC, 2011, O2k-Network
Prof. Dr.Thangaraj Kumarasamy: Centre for Cellular and Molecular Biology, Hyderabad, India. Email: firstname.lastname@example.org
The 64th Workshop on High-Resolution Respirometry (HRR) is the First Oxygraph-2k Workshop held in India. Participants are scientists, mostly the Ph.D. students, who want to learn in detail the experimental basis of high-resolution approaches to measure mitochondrial respiratory function. The workshop includes a basic introduction to the OROBOROS Oxygraph-2k with integrated on-line analysis by DatLab 4.3 and applications of the Titration-Injection microPump TIP2k. Specific emphasis is placed on on-line practical demonstration of instrument and software performance, to introduce the participants into the technical and operational details of high-resolution respirometry (HRR). Quality control of instrumental performance will be demonstrated by on-line calibration of the oxygen sensors, evaluation of signal stability, and perspectives of HRR in mitochondrial physiology.
Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols. An overview is given on the O2k-MultiSensor System, with the TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption. As an outlook to OROBOROS innovations, our newest perspectives will be presented on the O2k-Fluorescence Module, which is developed in the frame of the project MitoCom Tyrol. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.
O2k-Workshops are listed as MitoGlobal Events.