MiPNet16.02 IOC64

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MiPNet16.02 IOC64

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Hyderabad IN, 2001 December 08. O2k-Workshop on high-resolution respirometry - Satellite to 1st SMRM. OROBOROS IOC64. »IN Hyderabad Thangaraj K.

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OROBOROS, SMRM (2011-12-08) Mitochondr Physiol Network

Abstract: International Course on High-Resolution Respirometry - Satellite to 1st SMRM. Hyderabad, India; 2011 December 08

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O2k-Network Lab: IN Hyderabad Thangaraj K, AT_Innsbruck_OROBOROS


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HRR: Oxygraph-2k 

Additional: ORO, IOC, 2011, O2k-Network 


Contents


Contact

Prof. Dr.Thangaraj Kumarasamy: Centre for Cellular and Molecular Biology, Hyderabad, India. Email: thangs@ccmb.res.in


General information

The 64th Workshop on High-Resolution Respirometry (HRR) is the First Oxygraph-2k Workshop held in India. Participants are scientists, mostly the Ph.D. students, who want to learn in detail the experimental basis of high-resolution approaches to measure mitochondrial respiratory function. The workshop includes a basic introduction to the OROBOROS Oxygraph-2k with integrated on-line analysis by DatLab 4.3 and applications of the Titration-Injection microPump TIP2k. Specific emphasis is placed on on-line practical demonstration of instrument and software performance, to introduce the participants into the technical and operational details of high-resolution respirometry (HRR). Quality control of instrumental performance will be demonstrated by on-line calibration of the oxygen sensors, evaluation of signal stability, and perspectives of HRR in mitochondrial physiology.

Novel developments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols. An overview is given on the O2k-MultiSensor System, with the TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption. As an outlook to OROBOROS innovations, our newest perspectives will be presented on the O2k-Fluorescence Module, which is developed in the frame of the project MitoCom Tyrol. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.


IOC64.jpg

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