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Abstract: Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2011) NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. Mitochondr Physiol Network 08.12(07). »Versions
A single pilot experiment was carried out during an O2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Labels: MiParea: Respiration, Instruments;methods Mammal;model: Rat Tissue;cell: Liver Preparation: Isolated mitochondria Stress: Oxidative stress;RONS Regulation: Inhibitor, Oxygen kinetics Coupling state: OXPHOS HRR: Oxygraph-2k, NO, O2k-Protocol Additional: O2k-Demo, O2k-MultiSensor