MiPNet06.03 POS-calibration-SOP

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MiPNet06.03 POS-calibration-SOP

Publications in the MiPMap
O2k-Protocols
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

» Bioblast pdf

OROBOROS (2016-02-09) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(15):1-8. » Versions

O2k-Protocols SOP: High-resolution respirometry (HRR; OROBOROS Oxygraph-2k, O2k) critically depends on maintenance (MiPNet19.18B POS-service) and accurate calibration of the polarographic oxygen sensors (OroboPOS, POS). Standard operating procedures (O2k-SOP) are described as part of the MitoFit Quality Control System. (1) Clean and ready-to-use O2k-Chambers are a basic requirement (MiPNet19.03). (2) Quality control for evaluation of proper POS function (SOP: O2 sensor test). (3) Accurate POS calibration (MiPNet19.18D O2k-calibration). This is part 1 of O2k Quality Control.

» O2k-Protocols SOP: O2k Quality Control 2 »MiPNet14.06 Instrumental O2 background«
» O2k-Manual »MiPNet19.18D_O2k-calibration«
» Product: OroboPOS, O2k, O2k-Catalogue
» Template: File:O2-calibration.xlsx

O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods  HRR: Oxygraph-2k, O2k-Protocol Additional: O2k-SOP 


Further details

O2k-QCS Next step - O2k Quality Control 2: »MiPNet14.06 Instrumental O2 background«


cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.

References

This article is cited in
  • Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. »PubMed 18370948«
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