O2k-Workshops in O2k-Network Labs

From Bioblast

(Redirected from IOC in MiPNet Labs)
Jump to: navigation, search


OROBOROS INSTRUMENTS

OROBOROS         Products         Support         O2k-Publications         Workshops & Events         O2k-Network         Feedback         Company         Contact



O2k-Workshops in O2k-Network Labs

MiPNet Reference Laboratories

Contents

Concept

OROBOROS O2k-Workshops are organized in cooperation with O2k-Network Labs as a service for the O2k-Network Lab, and feedback support for OROBOROS INSTRUMENTS to disseminate the newest developments in the region by invitation to external participants.


O2k-Workshop programme

  1. Setting up the Oxygraph-2k and instrumental service.
  2. Protocols for high-resolution respirometry and O2k-Fluorometry: focus on combined measurement of respiration and mt-membrane potential.
  3. O2k-Demo experiment: Combined measurement of respiration and hydrogen peroxide production.
  4. Extending real-time data analysis to evaluation of results, discussion of protocol design, OROBOROS in science.


IOC in O2k-Network Labs

Date Location - Country - IOC
MiPNet21.03 IOC114 Cape Town ZA 2016-09-01
O2k-Network
Cape Town ZA, 2016 Sep 01-02. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry. O2k-Network Lab »ZA Cape Town Ojuka EO. IOC114.
MiPNet21.09 IOC113 Havana CU 2016-08-08
O2k-Network
Havana CU, 2016 Aug 08-09. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC113. O2k-Network Lab » CU Havana Pardo Andreu GL
MiPNet21.02 IOC111 Seattle WA US 2016-06-13
O2k-Network
Seattle WA US, 2016 Jun 13-14. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC111. O2k-Network Lab »US WA Seattle Marcinek DJ
MiPNet21.04 IOC110 Melbourne AU 2016-04-12
O2k-Network
Melbourne AU, 2016 Apr 12-13. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC110. O2k-Network Lab »AU Melbourne Stepto NK
MiPNet21.05 IOC108 Singapore SG 2016-03-06
O2k-Network
Singapore SG, 2016 Mar 06-07. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC108.
MiPNet21.08 IOC107 Vienna AT 2016-02-16
OROBOROS-solo.png
Vienna AT, 2016 Feb 16. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC107.
MiPNet20.16 IOC105 Riga 2015-08-27
O2k-Network
Riga LV, 2015 Aug 27. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - innovabalt, IOC105.
MiPNet20.05 IOC104 Greenville 2015-08-09
O2k-Network
Greenville NC US, 2015 Aug 09-10. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC104.
MiPNet20.12 IOC103 Manaus 2015-07-06
O2k-Network
Manaus BR, 2015 Jul 06-10. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.
MiPNet20.11 IOC102 Wuhan CN 2015-05-25
O2k-Network
Wuhan CN, 2015 May 25-26. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC102. OROBOROS representative in China: »CN Beijing Zenda.
MiPNet20.03 IOC101 London 2015-04-21
O2k-Network
London UK, 2015 Apr 21-23. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC101.
MiPNet20.02 IOC99 Cape Town 2015-03-23
O2k-Network
Cape Town ZA, 2015 Mar 23-24. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC99. - Satellite to 7th MiPschool Cape Town 2015.
MiPNet19.16 IOC98 2014-11-17
O2k-Network
Taipei TW, 2014 Nov 17-20. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC98.
MiPNet19.15 IOC97 2014-09-25
O2k-Network
Lausanne CH, 2014 Sep. 25-26 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC97.
MiPNet19.09 IOC94 Shanghai 2014-06-27
O2k-Network
Shanghai CN, 2014 Jun 27-Jul 02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC94. OROBOROS representative in China: »CN Beijing Zenda.
MiPNet19.06 IOC91 Philadelphia US 2014-06-02
O2k-Network
Philadelphia US, 2014 Jun 02-03. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC91. O2k-Network Lab »US PA Philadelphia Wallace DC.
MiPNet19.04 IOC89 2014-05-01
O2k-Network
La Jolla US, 2014 May 01-02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC89. O2k-Network Lab »US CA La Jolla Felding-Habermann B
MiPNet18.16 IOC87 Spitsbergen 2014-03-24
O2k-Network
Spitsbergen NO, 2014 Mar 24-27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry: Spitsbergen - Enjoy the Cold, IOC87. MiPNet Lab »NO Trondheim Rognmo O.
MiPNet18.15 IOC86 2014-01-31
O2k-Network
Mumbay IN, 2014 Jan 31. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC86. »IN Mumbai Kolthur-Seetharam U
MiPNet18.13 IOC84 Alaska 2014-01-04
O2k-Network
Big Lake, Alaska US, 2014 Jan 04-07. OROBOROS O2k-Workshop on HRR and Racing dogs at Iditarod, IOC84.  »US OK Stillwater Davis MS
MiPNet18.11 IOC82 2013-12-10
O2k-Network
Trondheim NO, 2013 Dec 10-11, OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, OROBOROS IOC82. Satellite to the 5th Seminar on Exercise in Medicine. Trondheim, Norway, O2k-Network Lab »NO Trondheim Rognmo O.
MiPNet18.20 IOC78 2013-08-27
O2k-Network
Copenhagen DK, 2013 Aug 27-29. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC78.
MiPNet18.04 IOC76 2013-05-28
O2k-Network
Prague CZ, 2013 May 28-29. O2k-Workshop on high-resolution respirometry, OROBOROS IOC76. O2k-Network Lab »CZ Prague Kalous M.
MiPNet18.01 IOC73 2013-01-15
O2k-Network
Kolkata IN, 2013 Jan 15-16. O2k-Workshop on high-resolution respirometry, OROBOROS IOC73. O2k-Network Lab »IN Kolkata Chakrabarti S.
MiPNet17.20 IOC71 2012-11-07
O2k-Network
Chongqing CN, 2012 Nov 07. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC71.
MiPNet17.10 IOC70 2012-05-29
O2k-Network
Barcelona ES, 2012 May 29-30. O2k-Workshop on high-resolution respirometry. OROBOROS IOC70.  »ES Barcelona Garcia-Roves PM.
MiPNet17.19 IOC69 2012-05-07
O2k-Network
Madrid ES, 2012 May 07. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC69.
MiPNet17.07 IOC67 2012-04-02
O2k-Network
Sidney AU, 2012 April 02-04. O2k-Workshop on high-resolution respirometry. OROBOROS IOC67. »AU Sydney Stocker R.
MiPNet16.02 IOC64 2011-12-08
O2k-Network
Hyderabad IN, 2001 December 08. O2k-Workshop on high-resolution respirometry - Satellite to 1st SMRM. OROBOROS IOC64. »IN Hyderabad Thangaraj K.
MiPNet16.05 IOC63 2011-08-26
O2k-Network
Beijing CN, 2011 Aug 26-27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC63.
MiPNet15.12 IOC57 2010-06-10
O2k-Network
Druskininkai LT, 2010 Jun 10- 16. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC57.
MiPNet14.08 IOC52 2009-06-21
O2k-Network
Baton Rouge US, 2009 Jun 21. Demo O2k-Workshop, IOC52 at MiPsummer 2009. O2k-Network Lab »US LA Baton Rouge Hand SC.
MiPNet14.02 IOC49 2009-02-23
O2k-Network
Gainsville US, 2009 Feb 23-25. International O2k-Workshop on high-resolution respirometry. OROBOROS IOC49.  »US FL Gainsville Univ Florida.
MiPNet12.04 IOC38 2007-03-03
O2k-Network
Rome IT, 2007 Mar 3. Workshop on high-resolution respirometry and mitochondrial physiology. OROBOROS IOC38, »IT Roma Sarti P
MiPNet12.03 IOC37 2007-02-04
O2k-Network
Seoul KR, 2007 Feb 4. Workshop on high-resolution respirometry and mitochondrial physiology. OROBOROS IOC37. »KR Seoul Lee HK.


MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.


O2k-Workshops and expeditions: environments at high altitude and cold

Oxygen delivery to the tissue is a critically limiting factor frequently studied in high altitude environments and uner hypothermia. Very few investigations, however, address the equally important metabolic process of cellular oxygen utilization under short-term and long-term hypobaric hypoxia. OROBOROS INSTRUMENTS is a highly competent potential partner of a multi-disciplinary and international research group focused on clinical and biological aspects of Human life at high altitude. We have participated in several expeditions to high altitude to study short-term acclimatization and adaptation of Human populations to high altitude and low temperature (Monte Rosa, Italy; Mt. Chacaltaya, Bolivia; Everest Base Camp, Nepal; Alaska, USA; Spitsbergen, Norway). We are specialists in instrumental development and applications in mitochondrial physiology and have taken high-resolution respirometry to the extremes of field work at high altitude, with long-term experience in the organization of workshops all-over the world. In methodological and technical workshops, participants are introduced to the conceptual and practical basis of the diagnosis of mitochondrial function and dysfunction. Specifically, the boundary conditions prevailing in laboratories available at high altitude can be taking into account, which require the highest performance standards for electronic instruments and their application. In addition we offer pre-expedition training to international partners in our research laboratory, for scientists and students to gain competence for the challenges met when working in high altitude environments. As a long-term perspective, we aim at elaborating standards for the evaluation of cell respiration and mitochondrial function to diagnose diseases and find potential targets to improve the state of health and quality of Human life in high altitude and hypothermic environments. These standards can be implemented not only in the participating countries but may become a template for world-wide dissemination.

Reference and links

  1. Previous expeditions: OROBOROS expeditions
  2. International workshops: OROBOROS O2k-Workshops
  3. OROBOROS Oxygraph-2k publications on hypoxia and mitochondrial function: O2k-Publications: Hypoxia

Selected publications on hypoxia and mitochondrial function

  1. Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.
  2. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
  3. Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.
  4. Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J (2008) Oxygen dependence of tyrosine hydroxylase. Amino Acids 34: 455-464.
  5. Aragonés J et al (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180.
  6. Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. Eur Respir J 36: 1056-1066.
  7. Smolková K, Bellance N, Scandurra F, Génot E, Gnaiger E, Plecitá-Hlavatá L, Ježek P, Rossignol R (2010) Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia. J Bioenerg Biomembr 42: 55-67.
  8. Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.
  9. Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
  10. Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
  11. Jacobs RA, Boushel RC, Wright-Paradis C, Calbet JA, Robach P, Gnaiger E, Lundby C (2013) Mitochondrial function in human skeletal muscle following high altitude exposure. Exp Physiol 98: 245-255.
Personal tools
Namespaces
Variants
Actions
OROBOROS
Bioblast
MitoGlobal
Support pages
Toolbox