O2k-Workshops in O2k-Network Labs

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O2k-Workshops in O2k-Network Labs

MiPNet Reference Laboratories

Concept

OROBOROS O2k-Workshops are organized in cooperation with O2k-Network Labs as a service for the O2k-Network Lab, and feedback support for OROBOROS INSTRUMENTS to disseminate the newest developments in the region by invitation to external participants.


O2k-Workshop programme

  • General: Focus on four sessions:
  1. Setting up the Oxygraph-2k and instrumental service.
  2. Protocols for high-resolution respirometry and O2k-Fluorometry: focus on combined measurement of respiration and mt-membrane potential.
  3. O2k-Demo experiment: Combined measurement of respiration and hydrogen peroxide production.
  4. Extending real-time data analysis to evaluation of results, discussion of protocol design, OROBOROS in science.
  • A detailed programme including the timing of sessions is developed in collaboration with the local host institution.


IOC in O2k-Network Labs

 DateLocation - Country - IOC
MiPNet21.09 IOC113 Havana CU2016-08-08
O2k-Network
Havana CU, 2016 Aug 08-09. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC113. O2k-Network Lab » CU Havana Pardo Andreu GL
MiPNet21.02 IOC111 Seattle WA US2016-06-13
O2k-Network
Seattle WA US, 2016 Jun 13-14. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC111. O2k-Network Lab »US WA Seattle Marcinek DJ
MiPNet21.04 IOC110 Melbourne AU2016-04-12
O2k-Network
Melbourne AU, 2016 Apr 12-13. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC110. O2k-Network Lab »AU Melbourne Stepto NK
MiPNet21.05 IOC108 Singapore SG2016-03-06
O2k-Network
Singapore SG, 2016 Mar 06-07. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC108.
MiPNet21.08 IOC107 Vienna AT2016-02-16
OROBOROS-solo.png
Vienna AT, 2016 Feb 16. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC107.
MiPNet20.16 IOC105 Riga2015-08-27
O2k-Network
Riga LV, 2015 Aug 27. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - innovabalt, IOC105.
MiPNet20.05 IOC104 Greenville2015-08-09
O2k-Network
Greenville NC US, 2015 Aug 09-10. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC104.
MiPNet20.12 IOC103 Manaus2015-07-06
O2k-Network
Manaus BR, 2015 Jul 06-10. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.
MiPNet20.11 IOC102 Wuhan CN2015-05-25
O2k-Network
Wuhan CN, 2015 May 25-26. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC102. OROBOROS representative in China: »CN Beijing Zenda.
MiPNet20.03 IOC101 London2015-04-21
O2k-Network
London UK, 2015 Apr 21-23. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC101.
MiPNet20.02 IOC99 Cape Town2015-03-23
O2k-Network
Cape Town ZA, 2015 Mar 23-24. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC99. - Satellite to 7th MiPschool Cape Town 2015.
MiPNet19.16 IOC982014-11-17
O2k-Network
Taipei TW, 2014 Nov 17-20. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC98.
MiPNet19.15 IOC972014-09-25
O2k-Network
Lausanne CH, 2014 Sep. 25-26 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC97.
MiPNet19.09 IOC94 Shanghai CN2014-06-27
O2k-Network
Shanghai CN, 2014 Jun 27-Jul 02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC94. OROBOROS representative in China: »CN Beijing Zenda.
MiPNet19.06 IOC91 Philadelphia US2014-06-02
O2k-Network
Philadelphia US, 2014 Jun 02-03. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC91. O2k-Network Lab »US PA Philadelphia Wallace DC.
MiPNet19.04 IOC89 La Jolla US2014-05-01
O2k-Network
La Jolla US, 2014 May 01-02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC89. O2k-Network Lab »US CA La Jolla Felding-Habermann B
MiPNet18.16 IOC87 Spitsbergen2014-03-24
O2k-Network
Spitsbergen NO, 2014 Mar 24-27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry: Spitsbergen - Enjoy the Cold, IOC87. MiPNet Lab »NO Trondheim Rognmo O.
MiPNet18.15 IOC862014-01-31
O2k-Network
Mumbay IN, 2014 Jan 31. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC86. »IN Mumbai Kolthur-Seetharam U
MiPNet18.13 IOC84 Alaska2014-01-04
O2k-Network
Big Lake, Alaska US, 2014 Jan 04-07. OROBOROS O2k-Workshop on HRR and Racing dogs at Iditarod, IOC84.  »US OK Stillwater Davis MS
MiPNet18.11 IOC822013-12-10
O2k-Network
Trondheim NO, 2013 Dec 10-11, OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry, OROBOROS IOC82. Satellite to the 5th Seminar on Exercise in Medicine. Trondheim, Norway, O2k-Network Lab »NO Trondheim Rognmo O.
MiPNet18.20 IOC782013-08-27
O2k-Network
Copenhagen DK, 2013 Aug 27-29. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC78.
MiPNet18.04 IOC762013-05-28
O2k-Network
Prague CZ, 2013 May 28-29. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC76. O2k-Network Lab »CZ Prague Kalous M.
MiPNet18.01 IOC732013-01-15
O2k-Network
Kolkata IN, 2013 Jan 15-16. O2k-Workshop on high-resolution respirometry, IOC73. O2k-Network Lab »IN Kolkata Chakrabarti S.
MiPNet17.20 IOC712012-11-07
O2k-Network
Chongqing CN, 2012 Nov 07. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC71.
MiPNet17.10 IOC702012-05-29
O2k-Network
Barcelona ES, 2012 May 29-30. OROBOROS O2k-Workshop on high-resolution respirometry (HRR), IOC70.  »ES Barcelona Garcia-Roves PM.
MiPNet17.19 IOC692012-05-07
O2k-Network
Madrid ES, 2012 May 07. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC69.
MiPNet17.07 IOC672012-04-02
O2k-Network
Sidney AU, 2012 Apr 02-04. OROBOROS O2k-Workshop on high-resolution respirometry, IOC67. »AU Sydney Stocker R.
MiPNet16.02 IOC642011-12-08
O2k-Network
Hyderabad IN, 2011 Dec 08. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) - Satellite to 1st SMRM, IOC64. »IN Hyderabad Thangaraj K.
MiPNet16.05 IOC632011-08-26
O2k-Network
Beijing CN, 2011 Aug 26-27. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC63.
MiPNet15.12 IOC57 Druskininkai LT2010-06-10
O2k-Network
Druskininkai LT, 2010 Jun 10- 16. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC57.
MiPNet14.08 IOC522009-06-21
O2k-Network
Baton Rouge US, 2009 Jun 21. Demo O2k-Workshop, IOC52 at MiPsummer 2009. O2k-Network Lab »US LA Baton Rouge Hand SC.
MiPNet14.02 IOC492009-02-23
O2k-Network
Gainsville US, 2009 Feb 23-25. OROBOROS O2k-Workshop on high-resolution respirometry (HRR), IOC49.  »US FL Gainsville Univ Florida.
MiPNet12.04 IOC382007-03-03
O2k-Network
Rome IT, 2007 Mar 03. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and mitochondrial physiology. OROBOROS IOC38, »IT Roma Sarti P
MiPNet12.03 IOC372007-02-04
O2k-Network
Seoul KR, 2007 Feb 04. OROBOROS O2k-Workshop on high-resolution respirometry (HRR) and mitochondrial physiology. OROBOROS IOC37. »KR Seoul Lee HK.


MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.


O2k-Workshops and expeditions: environments at high altitude and cold

Oxygen delivery to the tissue is a critically limiting factor frequently studied in high altitude environments and uner hypothermia. Very few investigations, however, address the equally important metabolic process of cellular oxygen utilization under short-term and long-term hypobaric hypoxia. OROBOROS INSTRUMENTS is a highly competent potential partner of a multi-disciplinary and international research group focused on clinical and biological aspects of Human life at high altitude. We have participated in several expeditions to high altitude to study short-term acclimatization and adaptation of Human populations to high altitude and low temperature (Monte Rosa, Italy; Mt. Chacaltaya, Bolivia; Everest Base Camp, Nepal; Alaska, USA; Spitsbergen, Norway). We are specialists in instrumental development and applications in mitochondrial physiology and have taken high-resolution respirometry to the extremes of field work at high altitude, with long-term experience in the organization of workshops all-over the world. In methodological and technical workshops, participants are introduced to the conceptual and practical basis of the diagnosis of mitochondrial function and dysfunction. Specifically, the boundary conditions prevailing in laboratories available at high altitude can be taking into account, which require the highest performance standards for electronic instruments and their application. In addition we offer pre-expedition training to international partners in our research laboratory, for scientists and students to gain competence for the challenges met when working in high altitude environments. As a long-term perspective, we aim at elaborating standards for the evaluation of cell respiration and mitochondrial function to diagnose diseases and find potential targets to improve the state of health and quality of Human life in high altitude and hypothermic environments. These standards can be implemented not only in the participating countries but may become a template for world-wide dissemination.

Reference and links

  1. Previous expeditions: The world as a laboratory
  2. International workshops: OROBOROS O2k-Workshops
  3. OROBOROS Oxygraph-2k publications on hypoxia and mitochondrial function: O2k-Publications: Hypoxia

Selected publications on hypoxia and mitochondrial function

  1. Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.
  2. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
  3. Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.
  4. Rostrup M, Fossbakk A, Hauge A, Kleppe R, Gnaiger E, Haavik J (2008) Oxygen dependence of tyrosine hydroxylase. Amino Acids 34: 455-464.
  5. Aragonés J et al (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180.
  6. Sommer N, Pak O, Schörner S, Derfuss T, Krug A, Gnaiger E, Ghofrani HA, Schermuly RT, Huckstorf C, Seeger W, Grimminger F, Weissmann N (2010) Mitochondrial cytochrome redox states and respiration in acute pulmonary oxygen sensing. Eur Respir J 36: 1056-1066.
  7. Smolková K, Bellance N, Scandurra F, Génot E, Gnaiger E, Plecitá-Hlavatá L, Ježek P, Rossignol R (2010) Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia. J Bioenerg Biomembr 42: 55-67.
  8. Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.
  9. Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
  10. Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
  11. Jacobs RA, Boushel RC, Wright-Paradis C, Calbet JA, Robach P, Gnaiger E, Lundby C (2013) Mitochondrial function in human skeletal muscle following high altitude exposure. Exp Physiol 98: 245-255.