Hepple 2012 Abstract Bioblast
|Hepple RT, Picard M, Taivassalo T (2012) Is latent mitochondrial dysfunction in aging muscle exposed through mitochondrial isolation? Mitochondr Physiol Network 17.12.|
Event: Bioblast 2012
Mitochondrial alterations are strongly implicated in aging, particularly that of post-mitotic tissues like skeletal muscle. To date the majority of studies have examined this issue using mechanically isolated mitochondria. Given the fragmentation of the native mitochondrial architecture induced by this approach, and the stress this imposes on the organelle (e.g., potentiating ROS emission and sensitivity to an apoptotic challenge ), it is important to consider whether mitochondrial isolation affects the assessment of alterations in mitochondrial function in aging skeletal muscle. To this end, we compared alterations in mitochondrial function (respiration, reactive oxygen species [ROS] emission, and function of the mitochondrial permeability transition pore [mPTP]) in aging skeletal muscle between isolated mitochondria and saponin-permeabilized myofibers, the latter representing a method that preserves mitochondrial architecture. Strikingly, we observed that routine mechanical isolation of mitochondria profoundly exaggerated the impact of aging on all indices of mitochondrial function, whereas permeabilized myofibers from aged muscles had no change in respiratory capacity, and relatively modest alterations in ROS emission and mPTP function . In addition to having important implications for our understanding of the severity of mitochondrial dysfunction in aging skeletal muscle, our results also suggest that vulnerabilities in aging mitochondria from skeletal muscle may be at least partially compensated for by the mitochondrial network structure in vivo and these vulnerabilities become unmasked following organelle isolation. The implications of these findings for our understanding of aging on mitochondrial function will be discussed.
- Picard M, Taivassalo T, Ritchie D, Wright KJ, Thomas MM, Romestaing C, Hepple RT (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6: e18317. Open Access
- Picard M, Ritchie D, Wright KJ, Romestaing C, Thomas MM, Rowan SL, Taivassalo T, Hepple RT (2010) Mitochondrial functional impairment with aging is exaggerated in isolated mitochondria compared to permeabilized myofibers. Aging Cell 9: 1032-1046.
• MiPNetLab: CA Montreal Hepple RT
Labels: MiParea: Respiration Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue, Isolated mitochondria Stress: Permeability transition, Oxidative stress;RONS Pathology: Aging;senescence Coupling state: LEAK, OXPHOS HRR: Oxygraph-2k
Russell T Hepple (1,2), Martin Picard (2), Tanja Taivassalo (2)
(1) McGill University Health Centre; Email: firstname.lastname@example.org
(2) Department of Kinesiology, McGill University, Montreal, QC, CANADA